Hieffclone
WebCN114107369A CN202411458461.XA CN202411458461A CN114107369A CN 114107369 A CN114107369 A CN 114107369A CN 202411458461 A CN202411458461 A CN 202411458461A CN 114107369 A CN114107369 A CN 114107369A Authority CN China Prior art keywords mcs pgl vector primer reaction Prior art date 2024-12-02 Legal status … Web本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成连接反应 …
Hieffclone
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Web16 de dez. de 2024 · The linear DNA was cyclized into ΔKlcA HS pHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) … Web2 de mai. de 2024 · Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the …
WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1.
Web1 de jun. de 2024 · this digested vector by a HieffClone Plus multi One-Step . Cloning Kit (10912ES10, Y esen, China). The T urboID . fragment was amplified by PCR from a commercial gene WebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status …
Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c‐Myc (L297A, A321del, L333A, R346A, S347A, E351A, V361A, and Q365A) were generated using Hieff Mut Site‐Directed Mutagenesis Kit (YEASEN, 11003ES10).
Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科 … the principle of contiguity involves:Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in … sigma gamma rho phoenix azWeb公司简介. 上海翊圣生物科技有限公司成立于2009年，总部设在上海，并且分别在北京、杭州、南京、武汉、广州（深圳）设有办事处。. 公司专注于酶制试剂和抗原抗体的开 … sigma gamma rho probate outfitsWeb15 de dez. de 2024 · Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of … sigma gamma rho poodle picshttp://www.labbase.net/Supply/SupplyItems-4428942.html sigma gamma rho powerpoint templateWeb产品描述. 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成 … sigma gamma rho powerpoint slide templateWeb丁香通为您提供HieffClone™P商品详情介绍：价格：￥410，货号：10912ES，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone™P商品详情页； 丁香通 丁香园 the principle of contiguity