Cisplatin 293t
WebMar 1, 2024 · HEK-293T (293T) and the human ovarian cancer cell lines, OVCAR3 and SKOV3, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). ... Despite the common use of cisplatin for the treatment of human ovarian cancer, resistance to cisplatin is a major issue in effective cancer treatment, which has driven us … WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... (#12259) packaging plasmids (all from Addgene Headquarters, Watertown, MA, USA), were co-transfected into 293T cells (ATCC; CRL-3216) by using Lipofectamine 2000 (Thermo …
Cisplatin 293t
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WebCisplatin is a major anticancer drug that kills cancer cells by damaging their DNA. Cancer cells cope with the drug by removal of the damages with nucleotide excision repair. We … WebCisplatin (DDP) is a common therapeutic option for non‐small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... packaging plasmids (all from Addgene Headquarters, Watertown, MA, USA), were co‐transfected into 293T cells (ATCC; CRL‐3216) by using Lipofectamine 2000 (Thermo Fisher Scientific ...
WebJun 3, 2013 · For pull-down of FLAG-tagged HSP90 proteins (FLAG-HSP90), 293T cells transfected with FLAG-tagged HSP90α or HSP90β were lysed in lysis buffer containing glycerol at 10% final concentration for cell lysate preparation, and 50 µl Affi-Gel 10 and 500 µl of Pt complex solution (5 mg Pt dissolved) were used to synthesize 2ClPt-agarose or …
WebOct 26, 2000 · Effect of Pyst1 expression on JNK and p38 activation by cisplatin. 293T cells were transiently transfected with the indicated amounts of c- myc -Pyst1 expression vector. Cells were then serum... WebOct 20, 2024 · Following MG132 treatment, ID2 accumulation was shown in 293T cells. To identify the effect of USP1 on ID2, 293T cells were transfected with USP1-carrying plasmid and analyzed by ID2 abundance. ... Furthermore, for further experiments, cisplatin-resistant samples were chosen by real-time polymerase chain reaction (RT-PCR) and Western …
WebJun 20, 2002 · Therefore, we next investigated whether cisplatin was able to modulate the FasL transcription. 293T cells were transiently transfected with a plasmid containing the FasL promoter joined to the luciferase gene as a reporter, and cells were treated for different times with cisplatin.
WebOct 25, 2016 · The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in … bitstore it-consulting gmbhWebIn the present study, TRIM23 was first screened by next-generation sequencing between the cisplatin (DDP)-resistant A549/DDP cell line and the parental A549 cell line, combined with integrated analysis of the Gene Expression Omnibus (GEO) … bitstorm itWebAug 16, 2024 · Platinum-based compounds, such as cisplatin or carboplatin, have been the most active clinical drug class for the treatment of a variety of solid tumors including cancers of the ovary, lung, and... bits to represent fractionsWebCisplatin is administered intravenously as short-term infusion in normal saline for treatment of solid and haematological malignancies. It is used to treat various types of cancers, … bit-storeWebMay 11, 2016 · Since 293T was the embryonic kidney cells, the fast clearance or neutralizations could have made them resistant towards DXR. In conclusion, the same degree of sensitivity from the 2D system did not translate to a 3D culture system, resulting in higher IC50 values than the 2D system. ... Chen CL, Wang CK, et al. Cisplatin-, … bits tornar mcWebCisplatin (CDDP) is a commonly used drug for gallbladder cancer (GBC) chemotherapy. However, resistance to CDDP treatment results in relapse. Therefore, there is a need for the development of... bit storesWebINTRODUCTION cis-Diaminedichloroplatinum (c-DDP, cisplatin) is a DNA- reactive agent commonly used in chemotherapy protocols in Induction of apoptosis is the primary mechanism of tumor the treatment of several kinds of human cancers. Lesions of cell killing by radiation and chemotherapeutic agents. bit storage ideas