WebDethiobiotin synthase is involved in biotin biosynthesis and catalyses the reaction (CO2 + 7,8-diaminononanoate + ATP = dethiobiotin + phosphate + ADP). The enzyme binds ATP (see motif in first 12 residues of the SEED alignment) and requires magnesium as a co-factor. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin] WebDec 14, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. …
Cloning, expression and characterization of histidine-tagged biotin ...
WebMay 1, 2016 · The function of biotin synthase is to convert dethiobiotin, a product of dethiobiotin synthase (encoded by bioD), the third enzyme in the biotin biosynthesis pathway, to biotin. Biotin is an indispensable nutrient found in all living cells. Microorganisms, plants and a few fungi (most fungi obtain it from the environment) are … WebAug 21, 2012 · DAPA synthase and dethiobiotin synthase are encoded by adjacent genes that can be alternatively spliced to specify a bifunctional fusion protein or separate enzymes (Muralla et al., 2008). The fusion and the separate dethiobiotin synthase are predicted to be mitochondrial and the separate DAPA synthase is predicted to be cytosolic; … opal the fairy
CDD Conserved Protein Domain Family: bioD - National Center …
WebDethiobiotin. The conversion of dethiobiotin (4) into biotin (5), which is catalyzed by biotin synthase, is the most novel step in biotin biosynthesis since it involves the formal … WebMycobacterium tuberculosis dethiobiotin synthase (MtDTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis. … WebJan 2, 2024 · Keto-aminopelargonic acid synthase, EC 2.3.1.47; diaminopelargonic acid synthase (aminotransferase), EC 2.6.1.62; dethiobiotin synthase, EC 6.3.3.3; and biotin synthase, EC 2.8.1.6. cyclizes by elimination of the phosphate group. The two amide bonds are thus formed using a single mole of ATP ^ ADP + Pj. opal therapeutics